16S Metagenomics Report

Sample: 10

Analysis software version: 2.5.1.3

Report Date: 09/08/2017 21:03:27

Sample Information

Sample ID:	plate1-B02
Sample Name:	10
Run Folder:	D:\Illumina\MiSeqAnalysis\170906_M00907_0076_000000000-BB7K4
Taxonomy File:	gg_13_5_species_32bp.dat

Sequencing Statistics

Total Reads	Reads Passing Quality Filtering	% Reads Passing Quality Filtering
16,796	9,998	59.5 %

Classification Rate Summary

Taxonomic Level	Reads Classified to Taxonomic Level	% Total Reads Classified to Taxonomic Level
Kingdom	9,727	97.29 %
Phylum	9,712	97.14 %
Class	9,562	95.64 %
Order	9,462	94.64 %
Family	9,456	94.58 %
Genus	8,510	85.12 %
Species	6,987	69.88 %

Sunburst Classification Chart

This sunburst chart shows the relative abundance of the classification results within each taxonomic level.
Click on any category to zoom in. Click the center button to zoom out.
Click "Reset Plot" to return to a Kingdom-level view.

Top 20 Classification Results by Taxonomic Level

This bar chart shows the relative abundance of the top 20 classification results within each taxonomic level.
Mouse over any category to see its description and abundance.

Classification Results by Taxonomic Level

Tables show the highest 8 taxonomic classifications at each level.
Pie charts show all classifications above 3.5% abundance.

Top Kingdom Classification Results

Classification	Number of Reads	% Total Reads
Bacteria	9,727	97.29 %
Unclassified at Kingdom level	271	2.71 %

Note: The "Other" category in this pie chart is the sum of all classifications with less than 3.5% abundance.

Top Phylum Classification Results

Classification	Number of Reads	% Total Reads
Proteobacteria	9,696	96.98 %
Unclassified at Phylum level	286	2.86 %
Actinobacteria	9	0.09 %
Firmicutes	7	0.07 %

Total Phylum-level Taxonomic Categories Identified: 4.
Note: The "Other" category in this pie chart is the sum of all classifications with less than 3.5% abundance.

Top Class Classification Results

Classification	Number of Reads	% Total Reads
Gammaproteobacteria	9,540	95.42 %
Unclassified at Class level	436	4.36 %
Actinobacteria	9	0.09 %
Epsilonproteobacteria	5	0.05 %
Bacilli	3	0.03 %
Betaproteobacteria	2	0.02 %
Clostridia	1	0.01 %
Deltaproteobacteria	1	0.01 %

Total Class-level Taxonomic Categories Identified: 9. This table shows the top 8 of 9 classifications.
Note: The "Other" category in this pie chart is the sum of all classifications with less than 3.5% abundance.

Top Order Classification Results

Classification	Number of Reads	% Total Reads
Enterobacteriales	9,352	93.54 %
Unclassified at Order level	536	5.36 %
Pasteurellales	25	0.25 %
Vibrionales	24	0.24 %
Pseudomonadales	19	0.19 %
Actinomycetales	9	0.09 %
Alteromonadales	7	0.07 %
Xanthomonadales	6	0.06 %

Total Order-level Taxonomic Categories Identified: 17. This table shows the top 8 of 17 classifications.
Note: The "Other" category in this pie chart is the sum of all classifications with less than 3.5% abundance.

Top Family Classification Results

Classification	Number of Reads	% Total Reads
Enterobacteriaceae	9,352	93.54 %
Unclassified at Family level	542	5.42 %
Pasteurellaceae	25	0.25 %
Vibrionaceae	24	0.24 %
Moraxellaceae	19	0.19 %
Thiotrichaceae	5	0.05 %
Campylobacteraceae	4	0.04 %
Xanthomonadaceae	4	0.04 %

Total Family-level Taxonomic Categories Identified: 23. This table shows the top 8 of 23 classifications.
Note: The "Other" category in this pie chart is the sum of all classifications with less than 3.5% abundance.

Top Genus Classification Results

Classification	Number of Reads	% Total Reads
Escherichia	7,064	70.65 %
Unclassified at Genus level	1,488	14.88 %
Serratia	783	7.83 %
Enterobacter	259	2.59 %
Klebsiella	168	1.68 %
Trabulsiella	93	0.93 %
Vibrio	23	0.23 %
Psychrobacter	16	0.16 %

Total Genus-level Taxonomic Categories Identified: 38. This table shows the top 8 of 38 classifications.
Note: The "Other" category in this pie chart is the sum of all classifications with less than 3.5% abundance.

Top Species Classification Results

Classification	Number of Reads	% Total Reads
Escherichia albertii	5,539	55.40 %
Unclassified at Species level	3,011	30.12 %
Serratia entomophila	778	7.78 %
Escherichia coli	150	1.50 %
Klebsiella variicola	130	1.30 %
Enterobacter hormaechei	116	1.16 %
Enterobacter amnigenus	84	0.84 %
Trabulsiella odontotermitis	62	0.62 %

Total Species-level Taxonomic Categories Identified: 46. This table shows the top 8 of 46 classifications.
Note: The "Other" category in this pie chart is the sum of all classifications with less than 3.5% abundance.