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16S Metagenomics Report


Sample: 1


Analysis software version: 2.5.1.3

Report Date: 09/08/2017 21:03:12



Sample Information

Sample ID:plate1-A01
Sample Name:1
Run Folder:D:\Illumina\MiSeqAnalysis\170906_M00907_0076_000000000-BB7K4
Taxonomy File:gg_13_5_species_32bp.dat

Sequencing Statistics

Total ReadsReads Passing Quality Filtering% Reads Passing Quality Filtering
19,32411,82461.2 %

Classification Rate Summary

Taxonomic LevelReads Classified to Taxonomic Level% Total Reads Classified to Taxonomic Level
Kingdom11,47097.01 %
Phylum11,44796.81 %
Class11,30395.59 %
Order11,20194.73 %
Family11,19794.70 %
Genus10,11385.53 %
Species8,37770.85 %


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Sunburst Classification Chart

This sunburst chart shows the relative abundance of the classification results within each taxonomic level.
Click on any category to zoom in. Click the center button to zoom out.
Click "Reset Plot" to return to a Kingdom-level view.

Top 20 Classification Results by Taxonomic Level

This bar chart shows the relative abundance of the top 20 classification results within each taxonomic level.
Mouse over any category to see its description and abundance.

Classification Results by Taxonomic Level

Tables show the highest 8 taxonomic classifications at each level.
Pie charts show all classifications above 3.5% abundance.

Top Kingdom Classification Results

ClassificationNumber of Reads% Total Reads
Bacteria11,47097.01 %
Unclassified at Kingdom level3542.99 %
Note: The "Other" category in this pie chart is the sum of all classifications with less than 3.5% abundance.

Image

Top Phylum Classification Results

ClassificationNumber of Reads% Total Reads
Proteobacteria11,41596.54 %
Unclassified at Phylum level3773.19 %
Actinobacteria190.16 %
Firmicutes60.05 %
Bacteroidetes30.03 %
Tenericutes10.01 %
Synergistetes10.01 %
Chrysiogenetes10.01 %
Total Phylum-level Taxonomic Categories Identified: 9. This table shows the top 8 of 9 classifications.
Note: The "Other" category in this pie chart is the sum of all classifications with less than 3.5% abundance.

Image

Top Class Classification Results

ClassificationNumber of Reads% Total Reads
Gammaproteobacteria11,26695.28 %
Unclassified at Class level5214.41 %
Actinobacteria190.16 %
Epsilonproteobacteria40.03 %
Clostridia40.03 %
Betaproteobacteria30.03 %
Alphaproteobacteria20.02 %
Synergistia10.01 %
Total Class-level Taxonomic Categories Identified: 12. This table shows the top 8 of 12 classifications.
Note: The "Other" category in this pie chart is the sum of all classifications with less than 3.5% abundance.

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Top Order Classification Results

ClassificationNumber of Reads% Total Reads
Enterobacteriales11,10093.88 %
Unclassified at Order level6235.27 %
Pasteurellales250.21 %
Actinomycetales190.16 %
Pseudomonadales140.12 %
Vibrionales110.09 %
Xanthomonadales50.04 %
Campylobacterales40.03 %
Total Order-level Taxonomic Categories Identified: 23. This table shows the top 8 of 23 classifications.
Note: The "Other" category in this pie chart is the sum of all classifications with less than 3.5% abundance.

Image

Top Family Classification Results

ClassificationNumber of Reads% Total Reads
Enterobacteriaceae11,10093.88 %
Unclassified at Family level6275.30 %
Pasteurellaceae250.21 %
Moraxellaceae130.11 %
Vibrionaceae110.09 %
Streptosporangiaceae80.07 %
Xanthomonadaceae50.04 %
Micromonosporaceae50.04 %
Total Family-level Taxonomic Categories Identified: 28. This table shows the top 8 of 28 classifications.
Note: The "Other" category in this pie chart is the sum of all classifications with less than 3.5% abundance.

Image

Top Genus Classification Results

ClassificationNumber of Reads% Total Reads
Escherichia8,28670.08 %
Unclassified at Genus level1,71114.47 %
Serratia9758.25 %
Enterobacter3392.87 %
Klebsiella2351.99 %
Trabulsiella1191.01 %
Sodalis310.26 %
Edwardsiella180.15 %
Total Genus-level Taxonomic Categories Identified: 46. This table shows the top 8 of 46 classifications.
Note: The "Other" category in this pie chart is the sum of all classifications with less than 3.5% abundance.

Image

Top Species Classification Results

ClassificationNumber of Reads% Total Reads
Escherichia albertii6,61355.93 %
Unclassified at Species level3,44729.15 %
Serratia entomophila9588.10 %
Klebsiella variicola1621.37 %
Escherichia coli1591.34 %
Enterobacter hormaechei1541.30 %
Enterobacter amnigenus1201.01 %
Trabulsiella odontotermitis840.71 %
Total Species-level Taxonomic Categories Identified: 45. This table shows the top 8 of 45 classifications.
Note: The "Other" category in this pie chart is the sum of all classifications with less than 3.5% abundance.

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